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Quanternary structure of multihexameric arthropod hemocyanins

Identifieur interne : 004327 ( Main/Exploration ); précédent : 004326; suivant : 004328

Quanternary structure of multihexameric arthropod hemocyanins

Auteurs : Marin Van Heel [Allemagne] ; Prakash Dube [Allemagne]

Source :

RBID : ISTEX:F7E3B16E7577D62797D9CAA91C4F98D5344A4C5E

English descriptors

Abstract

Abstract: Arthropod hemocyanins are large oligomeric oxygen-transporting proteins with molecular weight ranging from 450 kDa in the spiny lobster (Panulirus interruptus) up to more than 3.6 mDa in the horseshoe crab (Limulus polyphemus). Hemocyanins from different species consist of one or multiple copies of a hexameric building block (of 450 kDa) and are sufficiently large to be easily visualized in the electron microscope. Arthropod hemocyanins were among the first macromolecules studied by multivariate statistical image analysis techniques. We present an overview of the different characteristic molecular images of various multihexameric (1 × 6, 2 × 6, 4 × 6, and 8 × 6) assemblies as these occur in electron-microscopical preparations. We also model the different assemblies in three dimensions by merging multiple copies of the X-ray-diffraction electron density of the single hexameric hemocyanin of Panulirus interruptus. By making correct enantiomeric decisions while merging the densities at the various levels of assembly and by fine-tuning the assembly parameters used, a good match can be obtained between the microscopical images and two-dimensional projections calculated from the three-dimensional (3D) model densities. Knowledge of the quaternary structures of this intricate hierarchical family of oligomers is essential for understanding the allosteric interactions associated with their strong oxygen-binding cooperativity.

Url:
DOI: 10.1016/0968-4328(94)90007-8


Affiliations:


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Le document en format XML

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<term>Alignment procedures</term>
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<term>Amino</term>
<term>Amino acids</term>
<term>Androctonus</term>
<term>Androctonus australis</term>
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<term>Antigenic determinants</term>
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<term>Arrowhead domains</term>
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<term>Arthropod hemocyanins</term>
<term>Assembly parameters</term>
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<term>Biological macromolecules</term>
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<term>Californicum</term>
<term>Carbon foil</term>
<term>Carbon support</term>
<term>Carbon support film</term>
<term>Characteristic views</term>
<term>Chem</term>
<term>Class averages</term>
<term>Cleft</term>
<term>Cleft views</term>
<term>Cooperative oxygen binding</term>
<term>Correspondence analysis</term>
<term>Crustacean hemocyanins</term>
<term>Data cloud</term>
<term>Data compression</term>
<term>Decker</term>
<term>Density distribution</term>
<term>Different species</term>
<term>Different types</term>
<term>Different views</term>
<term>Direct contact</term>
<term>Dodecamer</term>
<term>Dodecamers</term>
<term>Dube</term>
<term>Early models</term>
<term>Electron densities</term>
<term>Electron density</term>
<term>Electron micrographs</term>
<term>Electron microscope</term>
<term>Electron microscopy</term>
<term>Enantiomeric</term>
<term>Euler</term>
<term>Euler angle</term>
<term>Euler angles</term>
<term>Eurypelma</term>
<term>Eurypelma californicum</term>
<term>Eurypelma californicum hemocyanin</term>
<term>Eurypelma hemocyanin</term>
<term>Flat hexameric half</term>
<term>Flip</term>
<term>Flip view</term>
<term>Flop</term>
<term>Flop view</term>
<term>Flop views</term>
<term>Grid</term>
<term>Handedness</term>
<term>Heel</term>
<term>Helix</term>
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<term>Hemocyanin molecule</term>
<term>Hemocyanin molecules</term>
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<term>Hemocyanins</term>
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<term>Hexameric</term>
<term>Hexameric molecule</term>
<term>Hexameric structure</term>
<term>Hexamers</term>
<term>Horseshoe crab</term>
<term>Image analysis</term>
<term>Image data</term>
<term>Image processing</term>
<term>Individual subunits</term>
<term>Interruptus</term>
<term>Invertebrate</term>
<term>Invertebrate dioxygen carriers</term>
<term>Invertebrate oxygen carriers</term>
<term>Keegstra</term>
<term>Lamy</term>
<term>Lesser extent</term>
<term>Leuven</term>
<term>Leuven university press</term>
<term>Limulus</term>
<term>Limulus hemocyanin</term>
<term>Limulus polyphemus</term>
<term>Limulus polyphemus hemocyanin</term>
<term>Linzen</term>
<term>Local axes</term>
<term>Long axis</term>
<term>Lower half</term>
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<term>Micrographs</term>
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<term>Model densities</term>
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<term>Molecule</term>
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<term>Multihexameric hemocyanins</term>
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<term>Outer surfaces</term>
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<term>Stable positions</term>
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<term>Subunit assignments</term>
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<term>Surface representation</term>
<term>Surface representations</term>
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<div type="abstract" xml:lang="en">Abstract: Arthropod hemocyanins are large oligomeric oxygen-transporting proteins with molecular weight ranging from 450 kDa in the spiny lobster (Panulirus interruptus) up to more than 3.6 mDa in the horseshoe crab (Limulus polyphemus). Hemocyanins from different species consist of one or multiple copies of a hexameric building block (of 450 kDa) and are sufficiently large to be easily visualized in the electron microscope. Arthropod hemocyanins were among the first macromolecules studied by multivariate statistical image analysis techniques. We present an overview of the different characteristic molecular images of various multihexameric (1 × 6, 2 × 6, 4 × 6, and 8 × 6) assemblies as these occur in electron-microscopical preparations. We also model the different assemblies in three dimensions by merging multiple copies of the X-ray-diffraction electron density of the single hexameric hemocyanin of Panulirus interruptus. By making correct enantiomeric decisions while merging the densities at the various levels of assembly and by fine-tuning the assembly parameters used, a good match can be obtained between the microscopical images and two-dimensional projections calculated from the three-dimensional (3D) model densities. Knowledge of the quaternary structures of this intricate hierarchical family of oligomers is essential for understanding the allosteric interactions associated with their strong oxygen-binding cooperativity.</div>
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