Quanternary structure of multihexameric arthropod hemocyanins
Identifieur interne : 004327 ( Main/Exploration ); précédent : 004326; suivant : 004328Quanternary structure of multihexameric arthropod hemocyanins
Auteurs : Marin Van Heel [Allemagne] ; Prakash Dube [Allemagne]Source :
- Micron [ 0968-4328 ] ; 1994.
English descriptors
- Teeft :
- 2fold axis, 6mer, Absolute handedness, Alignment, Alignment procedures, Allosteric, Amino, Amino acids, Androctonus, Androctonus australis, Androctonus australis hemocyanin, Antigenic determinants, Arrowhead, Arrowhead domains, Arthropod, Arthropod hemocyanins, Assembly parameters, Australis, Beintema, Bijlholt, Biol, Biological macromolecules, Boisset, Bonaventura, Bruggen, Californicum, Carbon foil, Carbon support, Carbon support film, Characteristic views, Chem, Class averages, Cleft, Cleft views, Cooperative oxygen binding, Correspondence analysis, Crustacean hemocyanins, Data cloud, Data compression, Decker, Density distribution, Different species, Different types, Different views, Direct contact, Dodecamer, Dodecamers, Dube, Early models, Electron densities, Electron density, Electron micrographs, Electron microscope, Electron microscopy, Enantiomeric, Euler, Euler angle, Euler angles, Eurypelma, Eurypelma californicum, Eurypelma californicum hemocyanin, Eurypelma hemocyanin, Flat hexameric half, Flip, Flip view, Flop, Flop view, Flop views, Grid, Handedness, Heel, Helix, Hemocyanin, Hemocyanin molecule, Hemocyanin molecules, Hemocyanin structure, Hemocyanins, Heterogeneity, Hexamer, Hexameric, Hexameric molecule, Hexameric structure, Hexamers, Horseshoe crab, Image analysis, Image data, Image processing, Individual subunits, Interruptus, Invertebrate, Invertebrate dioxygen carriers, Invertebrate oxygen carriers, Keegstra, Lamy, Lesser extent, Leuven, Leuven university press, Limulus, Limulus hemocyanin, Limulus polyphemus, Limulus polyphemus hemocyanin, Linzen, Local axes, Long axis, Lower half, Macromolecule, Main classes, Markl, Micrographs, Microscopy, Mirror symmetry, Model building, Model densities, Model projections, Model structure, Molec, Molecular images, Molecule, Multihexameric, Multihexameric hemocyanins, Multiple copies, Multivariate, Outer surfaces, Panulirus, Panulirus interruptus, Panulirus interruptus hemocyanin, Pentagonal, Pentagonal view, Physiol, Pixel, Polypeptide, Polyphemus, Present work, Projection, Projection direction, Projection directions, Quaternary, Quaternary structure, Reassembly, Reassembly experiments, Rectangular half, Reference images, Relative orientation, Ribosomal subunits, Right panel, Rocking angle, Rocking behavior, Schaick, Schutter, Side view, Single hexamer, Single hexamers, Sizaret, Spatial frequencies, Spiny lobster, Stable position, Stable positions, Staggering angle, Staining material, Stereo pair, Subunit, Subunit assignments, Subunit heterogeneity, Support film, Surface representation, Surface representations, Trimer, Ultramicroscopy, Upper half, Various subunits, Volbeda.
Abstract
Abstract: Arthropod hemocyanins are large oligomeric oxygen-transporting proteins with molecular weight ranging from 450 kDa in the spiny lobster (Panulirus interruptus) up to more than 3.6 mDa in the horseshoe crab (Limulus polyphemus). Hemocyanins from different species consist of one or multiple copies of a hexameric building block (of 450 kDa) and are sufficiently large to be easily visualized in the electron microscope. Arthropod hemocyanins were among the first macromolecules studied by multivariate statistical image analysis techniques. We present an overview of the different characteristic molecular images of various multihexameric (1 × 6, 2 × 6, 4 × 6, and 8 × 6) assemblies as these occur in electron-microscopical preparations. We also model the different assemblies in three dimensions by merging multiple copies of the X-ray-diffraction electron density of the single hexameric hemocyanin of Panulirus interruptus. By making correct enantiomeric decisions while merging the densities at the various levels of assembly and by fine-tuning the assembly parameters used, a good match can be obtained between the microscopical images and two-dimensional projections calculated from the three-dimensional (3D) model densities. Knowledge of the quaternary structures of this intricate hierarchical family of oligomers is essential for understanding the allosteric interactions associated with their strong oxygen-binding cooperativity.
Url:
DOI: 10.1016/0968-4328(94)90007-8
Affiliations:
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Le document en format XML
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<term>6mer</term>
<term>Absolute handedness</term>
<term>Alignment</term>
<term>Alignment procedures</term>
<term>Allosteric</term>
<term>Amino</term>
<term>Amino acids</term>
<term>Androctonus</term>
<term>Androctonus australis</term>
<term>Androctonus australis hemocyanin</term>
<term>Antigenic determinants</term>
<term>Arrowhead</term>
<term>Arrowhead domains</term>
<term>Arthropod</term>
<term>Arthropod hemocyanins</term>
<term>Assembly parameters</term>
<term>Australis</term>
<term>Beintema</term>
<term>Bijlholt</term>
<term>Biol</term>
<term>Biological macromolecules</term>
<term>Boisset</term>
<term>Bonaventura</term>
<term>Bruggen</term>
<term>Californicum</term>
<term>Carbon foil</term>
<term>Carbon support</term>
<term>Carbon support film</term>
<term>Characteristic views</term>
<term>Chem</term>
<term>Class averages</term>
<term>Cleft</term>
<term>Cleft views</term>
<term>Cooperative oxygen binding</term>
<term>Correspondence analysis</term>
<term>Crustacean hemocyanins</term>
<term>Data cloud</term>
<term>Data compression</term>
<term>Decker</term>
<term>Density distribution</term>
<term>Different species</term>
<term>Different types</term>
<term>Different views</term>
<term>Direct contact</term>
<term>Dodecamer</term>
<term>Dodecamers</term>
<term>Dube</term>
<term>Early models</term>
<term>Electron densities</term>
<term>Electron density</term>
<term>Electron micrographs</term>
<term>Electron microscope</term>
<term>Electron microscopy</term>
<term>Enantiomeric</term>
<term>Euler</term>
<term>Euler angle</term>
<term>Euler angles</term>
<term>Eurypelma</term>
<term>Eurypelma californicum</term>
<term>Eurypelma californicum hemocyanin</term>
<term>Eurypelma hemocyanin</term>
<term>Flat hexameric half</term>
<term>Flip</term>
<term>Flip view</term>
<term>Flop</term>
<term>Flop view</term>
<term>Flop views</term>
<term>Grid</term>
<term>Handedness</term>
<term>Heel</term>
<term>Helix</term>
<term>Hemocyanin</term>
<term>Hemocyanin molecule</term>
<term>Hemocyanin molecules</term>
<term>Hemocyanin structure</term>
<term>Hemocyanins</term>
<term>Heterogeneity</term>
<term>Hexamer</term>
<term>Hexameric</term>
<term>Hexameric molecule</term>
<term>Hexameric structure</term>
<term>Hexamers</term>
<term>Horseshoe crab</term>
<term>Image analysis</term>
<term>Image data</term>
<term>Image processing</term>
<term>Individual subunits</term>
<term>Interruptus</term>
<term>Invertebrate</term>
<term>Invertebrate dioxygen carriers</term>
<term>Invertebrate oxygen carriers</term>
<term>Keegstra</term>
<term>Lamy</term>
<term>Lesser extent</term>
<term>Leuven</term>
<term>Leuven university press</term>
<term>Limulus</term>
<term>Limulus hemocyanin</term>
<term>Limulus polyphemus</term>
<term>Limulus polyphemus hemocyanin</term>
<term>Linzen</term>
<term>Local axes</term>
<term>Long axis</term>
<term>Lower half</term>
<term>Macromolecule</term>
<term>Main classes</term>
<term>Markl</term>
<term>Micrographs</term>
<term>Microscopy</term>
<term>Mirror symmetry</term>
<term>Model building</term>
<term>Model densities</term>
<term>Model projections</term>
<term>Model structure</term>
<term>Molec</term>
<term>Molecular images</term>
<term>Molecule</term>
<term>Multihexameric</term>
<term>Multihexameric hemocyanins</term>
<term>Multiple copies</term>
<term>Multivariate</term>
<term>Outer surfaces</term>
<term>Panulirus</term>
<term>Panulirus interruptus</term>
<term>Panulirus interruptus hemocyanin</term>
<term>Pentagonal</term>
<term>Pentagonal view</term>
<term>Physiol</term>
<term>Pixel</term>
<term>Polypeptide</term>
<term>Polyphemus</term>
<term>Present work</term>
<term>Projection</term>
<term>Projection direction</term>
<term>Projection directions</term>
<term>Quaternary</term>
<term>Quaternary structure</term>
<term>Reassembly</term>
<term>Reassembly experiments</term>
<term>Rectangular half</term>
<term>Reference images</term>
<term>Relative orientation</term>
<term>Ribosomal subunits</term>
<term>Right panel</term>
<term>Rocking angle</term>
<term>Rocking behavior</term>
<term>Schaick</term>
<term>Schutter</term>
<term>Side view</term>
<term>Single hexamer</term>
<term>Single hexamers</term>
<term>Sizaret</term>
<term>Spatial frequencies</term>
<term>Spiny lobster</term>
<term>Stable position</term>
<term>Stable positions</term>
<term>Staggering angle</term>
<term>Staining material</term>
<term>Stereo pair</term>
<term>Subunit</term>
<term>Subunit assignments</term>
<term>Subunit heterogeneity</term>
<term>Support film</term>
<term>Surface representation</term>
<term>Surface representations</term>
<term>Trimer</term>
<term>Ultramicroscopy</term>
<term>Upper half</term>
<term>Various subunits</term>
<term>Volbeda</term>
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<front><div type="abstract" xml:lang="en">Abstract: Arthropod hemocyanins are large oligomeric oxygen-transporting proteins with molecular weight ranging from 450 kDa in the spiny lobster (Panulirus interruptus) up to more than 3.6 mDa in the horseshoe crab (Limulus polyphemus). Hemocyanins from different species consist of one or multiple copies of a hexameric building block (of 450 kDa) and are sufficiently large to be easily visualized in the electron microscope. Arthropod hemocyanins were among the first macromolecules studied by multivariate statistical image analysis techniques. We present an overview of the different characteristic molecular images of various multihexameric (1 × 6, 2 × 6, 4 × 6, and 8 × 6) assemblies as these occur in electron-microscopical preparations. We also model the different assemblies in three dimensions by merging multiple copies of the X-ray-diffraction electron density of the single hexameric hemocyanin of Panulirus interruptus. By making correct enantiomeric decisions while merging the densities at the various levels of assembly and by fine-tuning the assembly parameters used, a good match can be obtained between the microscopical images and two-dimensional projections calculated from the three-dimensional (3D) model densities. Knowledge of the quaternary structures of this intricate hierarchical family of oligomers is essential for understanding the allosteric interactions associated with their strong oxygen-binding cooperativity.</div>
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